Part:BBa_K4190015

EBP10 Half-life Extender

Extends half life of Exendin-4 in vivo.

Usage and Biology

A Half-life extender (elastin-based polypeptide) sequence was added to our insert on the C terminus in the S. cerevisiae gene fragment. The half-life extender is made up of a ten times repeat of the VPGVG amino acid sequence and has been shown to increase the half life of Exendin-4 by 3.7 fold [1]. This component of our protein will be utilized once we reach in vivo trials. The optimized codon sequence will produce our functional protein when transcribed, and the half-life extender should not interfere with the binding of Exendin-4 and the GLP-1 receptor.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] S. J. Jung et al., “In-vivo half-life and hypoglycemic bioactivity of a fusion protein of exenatide and elastin-based polypeptide from recombinant Saccharomyces cerevisiae,” J Biotechnol, vol. 303, pp. 16–24, Sep. 2019, doi:10.1016/j.jbiotec.2019.06.304.