Tag

Part:BBa_K4190002

Designed by: Zoe L. Petroff   Group: iGEM22_UCSC   (2022-09-19)


6xHis-Tag (E. coli)

Histidine tag attached to Exendin-4 to conduct immobilized metal affinity chromatography.

Usage and Biology

After the start codon for our desired gene code, the first element is the histidine tag (his-tag). Our his-tag is made up of six histidine amino acids. When translated, the his-tag will be connected to the N-terminus of our Exendin-4 protein. This allows us to verify that our desired protein was expressed through immobilized metal affinity chromatography [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] “His-Tag Purification | Chromatography Media, Columns & Kits | Bio-Rad.” https://www.bio-rad.com/featured/en/his-tag-purification.html (accessed Aug. 27, 2022).

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