Plasmid
Part:BBa_K4190000
Designed by: N/A Group: iGEM22_UCSC (2022-09-19)
pET28:GFP
Vector for gene insertion and subsequent protein expression.
Usage and Biology
For the expression of the protein Exendin-4 in E. coli, we used the pET28:GFP [1] plasmid as our backbone. This plasmid has a prokaryotic origin of replication and kanamycin antibiotic resistance. We inserted our gene fragment into the space currently taken up by Green Fluorescent Protein (GFP).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 192
Illegal XbaI site found at 959 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 192
Illegal NotI site found at 165 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 192
Illegal BglII site found at 1025
Illegal BamHI site found at 198
Illegal XhoI site found at 158 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 192
Illegal XbaI site found at 959 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 192
Illegal XbaI site found at 959
Illegal NgoMIV site found at 1057
Illegal NgoMIV site found at 2645
Illegal NgoMIV site found at 2805
Illegal NgoMIV site found at 5852 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 3725
References
[1] D. L. Shis and M. R. Bennett, “Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants,” Proc. Natl. Acad. Sci. U.S.A., vol. 110, no. 13, pp. 5028–5033, Mar. 2013, doi: 10.1073/pnas.1220157110.
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Categories
Parameters
//plasmidbackbone
None |