Part:BBa_K4189003
NOTOC__ PE3
Contents
Introduction
As we all know, plastic is not biodegradable. Indeed, it’s created from fossils such as crude oil and heated to create strong polymers that cannot be found in nature which results in micro-organisms that didn’t evolve to attack these strong and high energy-demanding bonds. That is why we tried to find and modify adherence proteins that bind our degradation system to the chosen plastics.
Usage and Biology
- it’s one peptide that is normally capable of binding to a PE plastic
The patent in which we found the peptides is the following one: Cunningham, S.D., Ford, C., Lowe, D.J., O’Brien, J.P., Wang, H., Square, K., and Wilkins, A.E. (54) POLYETHYLENE BINDING PEPTIDES AND METHODS OF USE. 55*
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
The promoter was characterized using GFP to measure gene expression at different concentrations and on various plastics to check its affinity and specificity. To do so, we inoculated E. Coli BL21 strain with pET GFP in LB-medium, and after inoculation, we induced and purified it in an Econocolumn Biorad.
The GFP was used as a tracker to be able to, during our adhesion tests to quantify the protein that adhered to the plastic by using a fluorometer.
Adherence tests
We couldn't make any tests on that peptide.
Results
Fluorometer result for PE3 :
| Elution (PE) | Droplet (PE) | Elution (PP) | Droplet (PP) | Replica (PE) | |
|---|---|---|---|---|---|
| Result | X | X | X | X | X |
| SDS alone | C1 3441 | C1 3408 | C1 3902 | C1. 2816 | C1. 2000 |
| GFP alone | C1. 4428 C2. 2640 | C1. 27549 C2. 523 | C1. 11 800 C2. 4288 | C1. 1130 C2. 317 | C1. 3998 C2. 3997 |
We couldn't use PE3
| None |