Coding

Part:BBa_K4188027

Designed by: Tobias Bröker   Group: iGEM22_WWU_Muenster   (2022-09-30)


SctHMGR for the cytosolic expression

The SctHMGR removes the limiting step in the mevalonate pathway the conversion from HMG-CoA to mevalonate. Truncated version without repression side. The gene is MoClo (RFC1000) compatible.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1236
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 610
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1236
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1236
    Illegal NgoMIV site found at 1381
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None