Coding

Part:BBa_K4188023

Designed by: Tobias Bröker   Group: iGEM22_WWU_Muenster   (2022-09-30)


AgtCS for the cytosolic expression

The Abies grandis truncated camphene synthase catalyzes the reaction from GPP to camphene, a high-value monoterpenoid. It is missing the chloroplast tag, for efficient expression, folding, and function within the cytosol. The gene is MoClo (RFC1000) compatible.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 751
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 751
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 751
    Illegal BamHI site found at 1023
    Illegal XhoI site found at 1103
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 751
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 751
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None