Coding
Part:BBa_K4188023
Designed by: Tobias Bröker Group: iGEM22_WWU_Muenster (2022-09-30)
AgtCS for the cytosolic expression
The Abies grandis truncated camphene synthase catalyzes the reaction from GPP to camphene, a high-value monoterpenoid. It is missing the chloroplast tag, for efficient expression, folding, and function within the cytosol. The gene is MoClo (RFC1000) compatible.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 751
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 751
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 751
Illegal BamHI site found at 1023
Illegal XhoI site found at 1103 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 751
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 751
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |