Part:BBa_K4188003

pSB3CY_Leu shuttle vector with leucine synthase

This is the ready-to-use version of the pSB3CY_shuttlevektor backbone (BBa_K4188001) for Saccharomyces cerevisiae where the aeBlue chromoprotein is exchanged with the leucine synthase LEU2.It can be used for MoClo like the pSB3C01 backbone.

Test transformation

After a successful golden gate exchange of the aeBlue chromoprotein through the leucine synthase, the resulting vector pSB3CY_Leu was transformed into the yeast Saccharomyces cerevisiae.

Figure 1: left picture: Saccharomyces cerevisiae colonies formed from 100µl plated transformation mixture
right picture: Saccharomyces cerevisiae colonies formed from 10µl plated from the same transformation mixture

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 7094
  Illegal XbaI site found at 3425
  Illegal SpeI site found at 3790
  Illegal PstI site found at 1105
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 7094
  Illegal NheI site found at 5780
  Illegal SpeI site found at 3790
  Illegal PstI site found at 1105
  Illegal NotI site found at 5721
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 7094
  Illegal BamHI site found at 2441
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 7094
  Illegal XbaI site found at 3425
  Illegal SpeI site found at 3790
  Illegal PstI site found at 1105
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 7094
  Illegal XbaI site found at 3425
  Illegal SpeI site found at 3790
  Illegal PstI site found at 1105
  Illegal AgeI site found at 793
  Illegal AgeI site found at 905
  Illegal AgeI site found at 2083
  Illegal AgeI site found at 2406
  Illegal AgeI site found at 4697
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1378
  Illegal BsaI site found at 7100
  Illegal BsaI.rc site found at 1099
  Illegal SapI site found at 1082
  Illegal SapI.rc site found at 6