Translational_Unit

Part:BBa_K4187021

Designed by: Martin Pezous   Group: iGEM22_Ionis_Paris   (2022-10-07)


Pblind-RBS-PLAase-T1-T7TE

PLAase is a polylactate depolymerase from the bacterium Rhodopseudomonas palustris that catalyzes the reaction of polylactate into lactate. Under the control of the Pblind promoter, its transcription is regulated by the EL222 protein (BBa_K2332004).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 152
    Illegal NgoMIV site found at 190
    Illegal NgoMIV site found at 199
    Illegal NgoMIV site found at 425
    Illegal AgeI site found at 224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 685


[edit]
Categories
Parameters
None