Translational_Unit

Part:BBa_K4187020

Designed by: Martin Pezous   Group: iGEM22_Ionis_Paris   (2022-10-07)


T7-RBS-EL222-T1-T7TE

EL222 is a natural photosensitive DNA-binding protein from the marine bacterium Erythrobacter litoralis HTCC2594. In the dark, EL222 is in its inactive form where its N-terminal LOV domain represses its DNA-binding C-terminal HTH domain. Upon blue light exposure (450nm), LOV-HTH interaction is released, allowing it to dimerize and bind its DNA binding regions. In our system, this protein will regulate the transcription of the PLA depolymerase (BBa_K4187021)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 563
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 133
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None