Composite

Part:BBa_K4186002

Designed by: Elijah Boateng   Group: iGEM22_AshesiGhana   (2022-10-07)


Arsenic Inducer

General Information

It senses the presence of arsenic in the environment and then induces a yellow glow in its presence.


Usage and Biology

Using an inducible promoter comes with shortcomings such as high leakage, low induced fold change, and poor sensitivity. Hence, the team utilized a constitutive promoter regulated by the ArsR regulatory gene. Given that the constitutive promoter is always active independent of the transcription factors, in the absence of arsenite (As3+), ArsR binds to the RBS to repress Pars promoter transcription. However, in the presence of As3+, arsR protein binds with the arsenite, triggering the reporter gene (amilGFP) expression resulting in the yellow glow of the bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 353
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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