Composite

Part:BBa_K4186001

Designed by: Elijah Boateng   Group: iGEM22_AshesiGhana   (2022-10-07)


Ferric inducer

General Information

It induces the presence of a red glow in the presence of iron.


Usage and Biology

This module consists of a constitutive promoter Pcat which is by default independent of the transcription factor. The FUR protein acts as a repressor for the PAceB and is always produced. In the absence of Fe2+, the PAceB is turned on, allowing for the transcription, resulting in the TetR binding repressing the Ptet, inhibiting further transcription, therefore the reporter gene is not expressed. However, in the presence of Fe2+ , the FUR protein binds with the ions, repressing the PAceB hence the TetR protein won’t be produced and Ptet is turned on allowing for the reporter gene eforRed to be expressed, giving out a pink glow.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 648
    Illegal BamHI site found at 926
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 305


[edit]
Categories
Parameters
None