Composite

Part:BBa_K4186000

Designed by: Elijah Boateng   Group: iGEM22_AshesiGhana   (2022-10-07)


Kill Switch

General Information

It terminates the life of the bacteria.

Usage and biology

To ensure no escape from the predetermined surroundings, the kill switch gene is crucial for eradicating the bacteria that might have escaped from our device. A T4 endolysin and a UV promoter make up our kill switch. The UV promoter is an inducible promoter that is regulated by UV irradiation. When the peptidoglycan layer of the E. coli is degraded, the T4 endolysin can release lysozyme, which causes cell lysis and kills the bacteria, preventing it from interacting with other species in the environment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 375
    Illegal AgeI site found at 445
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None