Part:BBa_K4181026
Dsup- K198M_V195R
We directed the evolution of the DSUP protein to convert lysine at position 198 to methionine and valine at position 195 to arginine.
Viability Test
After verification of protein expression, we next turn to characterize the proteins and test their functions under the target conditions of UV radiations. The first experiments was test on Dsup protein. This protein is found to bind the genome by its relatively flexible sequence. We firstly induced target protein expression in the three B.subilis groups (as mentioned under the subtitles of sporulation) and spreaded them on the plate. Next, the plates were exposed to a grandient of UV intensities and then cultured under 37℃ overnight. Bacteria survival rate was then analysed by counting colony numbers and make comparison between our results. The photoes taken for our 45 plates and the statistical analyses are showing below.</p>
Figure 3.Pictures of 45 plates(9*5) and statistical results.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 515
Illegal PstI site found at 1139 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 515
Illegal PstI site found at 1139 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 163
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 515
Illegal PstI site found at 1139 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 515
Illegal PstI site found at 1139
Illegal AgeI site found at 121 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 197
| None |