Regulatory
Part:BBa_K4180001
Designed by: HuiChing Kuo Group: iGEM22_KCIS_Xiugang_Taipei (2022-07-09)
pGal promoter
Gal promoter is in the plasmid and genes are cloned downstream of the pGal promoter, so the cloned genes can be induced in the presence of galactose
Galactose promoter can be induced in the presence of galactose and suppressed in the presence of glucose
Citation: Investigations in Molecular Cell Biology (O'Connor); 13.1: Regulation of the GAL1 promoter;
<https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/13%3A_Protein_overexpression/13.01%3A_Regulation_of_the_GAL1_promoter>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 286
- 1000COMPATIBLE WITH RFC[1000]
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 286
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
| None |