Part:BBa_K4180000

SNF1 is the metabolism sensor

AMP is an energy sensor regulated by energy depletion, such as AMP (adenosine monophosphate): ATP (adenosine triphosphate) ration is high, AMPK is induced by upstream pathway to act as the master regulator of energy metabolism, it will increase catabolic process, such as triggering fatty acid catabolism for more ATP, and reduce anabolic process, such as protein and fatty acid synthesis to reduce energy consumed (Herzig and Shaw 2018)
SNF1in yeast is homology of AMPKα in human, which is a major metabolism sensor protein of the whole metabolic pathways, increasing catabolic process, such as triggering fatty acid catabolism for more ATP, and reducing anabolic process, such as protein and fatty acid synthesis to reduce energy consumed

Citation: Herzig, S. and R. J. Shaw (2018). "AMPK: guardian of metabolism and mitochondrial homeostasis." Nat Rev Mol Cell Biol 19(2): 121-135.

Sequence and Features

      BBa_K4180000 SNF1 full length
          PCR product

After Bacteria transformation, bacteria colonies were picking up directly to do SNF1 full length PCR - colonies #1, 3, 5, and 6 had BBa_K4180000(SNF1           full length)
         PCR product

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 528
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]