Part:BBa_K4179021

cTNT promoter

It is a fragment from the chicken troponin muscle-specific gene [1]. Usually, it is found downstream to GTIIC motifs that are required for efficient expression [2].

Usage and Biology

The team of Technion 2022 used this promoter in a construct (BBa_K4179020) downstream to the 8XGTIIC motifs (BBa_K4179017) and followed by the luciferase gene (BBa_I712019). The whole composite including cTNT promoter plays a significant role in the OraCell measurement that has been created in order to quantify their manufactured product. Visit their wiki site to read more.

References

1.Mar, J. H., Antin, P. B., Cooper, T. A., & Ordahl, C. P. (n.d.). Analysis of the Upstream Regions Governing Expression of the Chicken Cardiac Troponin T Gene in Embryonic Cardiac and Skeletal Muscle Cells. Retrieved from http://rupress.org/jcb/article-pdf/107/2/573/1056686/573.pdf

2.Tidyman, W. E., Sehnert, A. J., Huq, A., Agard, J., Deegan, F., Stainier, D. Y. R., & Ordahl, C. P. (2003). In vivo regulation of the chicken cardiac troponin T gene promoter in zebrafish embryos. Developmental Dynamics, 227(4), 484–496. doi: 10.1002/dvdy.10328

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 71
  
• 1000
  COMPATIBLE WITH RFC[1000]