DNA
Part:BBa_K4177006
Designed by: Junchen Dong Group: iGEM22_HiZJU-China (2022-10-08)
dCas9
dCas9 protein is a mutant of Cas9 protein, in which two nuclease active regions, RuvC1 and HNH, are mutated at the same time. Thus, the endonuclease activity of the dCas9 protein is completely eliminated, leaving only the ability to be guided into the genome by the gRNA.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1340
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1340
Illegal NheI site found at 1099 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1340
Illegal BamHI site found at 3378 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1340
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1340
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |