DNA

Part:BBa_K4177001

Designed by: Yuyan Chen   Group: iGEM22_HiZJU-China   (2022-10-08)


HarmOR10

G protein-coupled receptor (GPCR) HarmOR10 is one of the odorant receptors (ORs) isolated from Helicoverpa armigera, which is specific for benzaldehyde. In the project, we plan to introduce the encoding gene of HarmOR10 from Helicoverpa armigera into engineering yeasts (S. cerevisiae). Simultaneously, the original GPCR of the yeast α-factor receptor (Ste2) will be knocked out. When tea plants infested by tea aphids thus release benzaldehyde into the air, HarmOR10 would recognize the signal and evoke further intracellular GPCR pathways in yeast.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
    Illegal XhoI site found at 1180
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None