Composite

Part:BBa_K4175055

Designed by: Chentao Li   Group: iGEM22_ZJUintl-China   (2022-10-07)


CAR-COSMO

We inserted the sequence of COSMO into the structure of CAR in expectation of forming a new synthetic protein. We inserted the COSMO sequence between 4-1BB and CD3ζ sequences in the hope that COSMO could block the signal of 4-1BB activation while COSMO sequence could activate its downstream CD3ζ when caffeine is present. where the CAR sequence was provided by Mr. Ruirui Jing of He Huang's group at Zhejiang University and the sequence was checked for correct validity.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1227
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1227
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1227
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1227
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1227
    Illegal NgoMIV site found at 899
    Illegal NgoMIV site found at 1692
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 444
    Illegal BsaI.rc site found at 732


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Categories
Parameters
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