Coding

Part:BBa_K4175052

Designed by: Chentao Li   Group: iGEM22_ZJUintl-China   (2022-09-26)


Caffeine-operated synthetic module (COSMO)

A genetically encoded caffeine manipulation synthesis module (COSMO) serves as a powerful chemically induced dimerization (CID) system.COSMO is capable of chemically genetically manipulating biological processes via caffeine and its metabolites as well as caffeine-containing beverages including coffee, tea, soda, and energy drinks. This CID tool evolved from caffeine-resistant nanosomes through a cell-based high-throughput screen. Further rationalized engineering of COSMO leads to 34-217-fold enhancement in caffeine sensitivity (EC50 = 16.9 We fused the protein to the chimeric antigen receptor in the hope that the protein could block the signal from the single-chain fragment variable while activating its downstream signal in the presence of caffeine


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 136
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 136
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 136
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 136
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 136
  • 1000
    COMPATIBLE WITH RFC[1000]


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