Part:BBa_K4175051
anti-DPP4 CAR->Jurkat cells
The anti-DPP4 CAR gene encodes the chimeric antigen receptor specific to the DPP4 protein. This gene is designed to be transfected into Jurkat cells which will become CAR-T cells expressing anti-DPP4 CAR, so the transfected Jurkat cells can target and clear the cells expressing the membrane-bound DPP4 protein. DPP4 was a potential senescent marker which selectively expressed on the surface of senescent, but not proliferating, human diploid fibroblasts. We engineered an anti-DPP4 CAR, containing anti-DPP4 scFv, CD8 hinge, CD8 transmembrane domain, 4-1BB co-stimulator domain, and CD3-ζ; domain. When the engineered CAR-T cells recognize the DPP4 highly expressed senescent cells, the downstream 4-1BB and CD3-ζ; will be activated to promote the proliferation of T cells and secretion of granzymes and perforins to achieve the goal of killing the senescent cells.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 619
Illegal PstI site found at 269
Illegal PstI site found at 1231 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 70
Illegal PstI site found at 269
Illegal PstI site found at 1231 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 342
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 619
Illegal PstI site found at 269
Illegal PstI site found at 1231 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 619
Illegal PstI site found at 269
Illegal PstI site found at 1231
Illegal NgoMIV site found at 871 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1321
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