Coding

Part:BBa_K4175050

Designed by: Zhengxun Li   Group: iGEM22_ZJUintl-China   (2022-09-22)


anti-DPP4 scFv

DPP4 was a potential senescent marker which selectively expressed on the surface of senescent, but not proliferating, human diploid fibroblasts. The anti-DPP4 scFv can binds to the DPP4 on the cell surface and activates the downstream 4-1BB domain and CD3-ζ domain to activate the T cells to clear the senescent cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 619
    Illegal PstI site found at 269
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 70
    Illegal PstI site found at 269
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 342
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 619
    Illegal PstI site found at 269
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 619
    Illegal PstI site found at 269
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None