Part:BBa_K4175006

4-1BB

4-1BB is a costimulatory receptor on activated T cells. The ligation of 4-1BB will trigger a signal cascade, which leads to activation of anti-apoptotic molecules, increased cytokine secretion, and enhanced cytotoxicity (Chester et al., 2018). It has been widely used in the second-generation CAR and is found to improve the persistence of CAR-T cells by enhancing CAR-T survival and expansion through non-canonical NFκB signaling pathway (Philipson et al., 2020). In our project, 4-1BB is used in anti-CD19 CAR (BBa_K4175012).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Chester, C., Sanmamed, M.F., Wang, J., Melero, I., 2018. Immunotherapy targeting 4-1BB: mechanistic rationale, clinical results, and future strategies. Blood 131, 49–57. https://doi.org/10.1182/blood-2017-06-741041

Philipson, B.I., O’Connor, R.S., May, M.J., June, C.H., Albelda, S.M., Milone, M.C., 2020. 4-1BB costimulation promotes CAR T cell survival through noncanonical NF-κB signaling. Sci. Signal. 13, eaay8248. https://doi.org/10.1126/scisignal.aay8248