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Part:BBa_K4173042

Designed by: Haoyu Sun   Group: iGEM22_NJU-China   (2022-10-09)


CMV ehancer->CMV promoter->Piezo1-siRNA-5

This is a functional unit to produce the Piezo1-siRNA. Piezo1 is a mechanically gated cation channel in cell membranes. Piezo1 has been used for the treatment of weightlessness related diseases. According to literature review, Piezo1 is also abnormally activated in the process of apoptosis of hair follicle stem cells, and inhibiting the function of Piezo1 receptor can alleviate the apoptosis of hair follicle stem cells caused by mechanical stress. Meanwhile, up-regulation of apoptosis in hair follicle stem cells was also observed during androgen alopecia. Our siRNA can inhibit the translation of Piezo1 gene by binding to its mRNA, thereby down-regulating the level of Piezo1 receptor on cell membrane and inhibiting the apoptosis of hair follicle stem cells during androgen alopecia.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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