Part:BBa_K4173024
L7Ae
L7Ae is an archaeal ribosomal protein 7Ae 60S large ribosomal subunit, a subunit of archaeal RNase P, plays multiple roles in archaea. It is part of the H/ACA and the C/D box snoRNPs, which catalyze rRNA pseudouridylation and 2’-O-methylation respectively. Thus, L7Ae is one of the most commonly used riboswitches which RNA-only delivery gene circuits highly rely on except RNA binding proteins (RBP). L7Ae, a K-turn and K-loop binding protein, is a component of box C/D RNPs and interacts with the K-turn motifs of archaeal box C/D sRNAs3. Through the interaction between L7Ae and C/D box RNA structure which is added at UTR of reporter gene, the target protein can be grasped and stay close to L7Ae. Then target protein can be held on the membrane of exosomes when L7Ae is conjugated to the C-terminus of CD63. Finally, L7Ae-CD63 can serve as an active packaging device of specific RNAs into exosomes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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