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Part:BBa_K4170090
lacI promoter
LacI promoter which regulates the transcription of LacI repressor
Usage and Biology
The lac repressor, LacI, is a DNA-binding transcription factor that normally represses the transcription of the operon which is involved in the catabolism of lactose. In this repression system, LacI binds to two operators, and formation of the repressor loop is critical (Egan and Schleif, 1993). This repressor binds in tandem to inverted repeat sequences that are 21 nucleotides long and possess conserved motifs. When the physiological inducer allolactose binds to the lac repressor prevents it from binding to the operator and induction occurs. IPTG which is a β-thiogalactosidase nonphysiological and nonphysiological analog, can also induce the transcription (Perros et al., 1996) .
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Citations
[1] Egan, S. and Schleif, R., 1993. A Regulatory Cascade in the Induction of rhaBAD. Journal of Molecular Biology, 234(1), pp.87-98.
[2] Perros, M., Steitz, T., Fried, M., Hudson, J. and Lewis, M., 1996. DNA Looping and Lac Repressor-CAP Interaction. Science, 274(5294), pp.1929-1932.
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