Device

Part:BBa_K417006

Designed by: Benjamin Porebski   Group: iGEM10_Monash_Australia   (2010-10-26)

LacI ethylene generator

LacI regulated ethylene generator that expresses the enzymes SAM Synthetase (BBa_K417000), ACC Synthase (BBa_K417001) and ACC Oxidase (BBa_K417002).

All enzymes are under the strongest ribosome site (BBa_B0034)

Finally the generator mRNA is terminated by BBa_B0010 and BBa_B0012.

The overall function of this generator is to produce Ethylene from L-Methionine in the presence of ATP and Ascorbic Acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2093
    Illegal BamHI site found at 328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 908
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/signalling/sender
//function/biosynthesis
Parameters
None