Part:BBa_K4170052
rhaS native derived from E.coli
DNA-binding transcriptional activator for rhaBAD and rhaT, L-rhamnose-binding
Usage and Biology
The "Rhamnose regulator," RhaS, is a transcription factor involved in L-rhamnose degradation and transport. RhaS alone is able to activate transcription of rha operons, but in the presence of CRP, transcription increases. The synthesis of rha operons is induced when E. coli is grown on L-rhamnose in the absence of glucose and when cellular cyclic AMP levels are high.RhaS is part of the unusual rhaSR operon that encodes two transcriptional regulators, RhaS and RhaR, both members of the AraC/XylS family of transcriptional regulators (Kolin, Jevtic, Swint-Kruse and Egan, 2007). The expression of operons involved in transport and degradation of L-rhamnose firstly requires the expression of RhaR, which induces transcription of the rhaSR operon. In this way, RhaS activates transcription of L-rhamnose gene clusters.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 393
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 661
- 1000COMPATIBLE WITH RFC[1000]
=Citations
[1] Egan, S. and Schleif, R., 1993. A Regulatory Cascade in the Induction of rhaBAD. Journal of Molecular Biology, 234(1), pp.87-98.
[2] Kolin, A., Jevtic, V., Swint-Kruse, L. and Egan, S., 2007. Linker Regions of the RhaS and RhaR Proteins. Journal of Bacteriology, 189(1), pp.269-271.
[2] Tobin, J. and Schleif, R., 1990. Purification and properties of RhaR, the positive regulator of the l-rhamnose operons of Escherichia coli. Journal of Molecular Biology, 211(1), pp.75-89.
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