Part:BBa_K4170051
rhaR native derived from E.coli
DNA-binding transcriptional activator for rhaSR, L-rhamnose-binding
Usage and Biology
The "Rhamnose regulator," RhaR, is a transcription factor that regulates genes involved in L-rhamnose degradation and coregulates with the global transcriptional regulator CRP. These regulators bind cooperatively to activate transcription of the unusual operon rhaSR, which encodes two transcriptional regulators, RhaS and RhaR , both members of the AraC/XylS family of transcriptional regulators and involved in L-rhamnose transport and degradation(Egan and Schleif, 1993). .
The expression of the operons involved in transport and degradation of L-rhamnose first requires the expression of RhaR, which induces transcription of the rhaSR operon. In this way, RhaS activates transcription of L-rhamnose gene clusters. When E. coli is grown on L-rhamnose in the absence of glucose, the transcription of the rhaSR is induced. Gene induction occurs when the physiological inducer, L-rhamnose, binds to RhaR and when cellular cyclic AMP levels are high(Tobin and Schleif, 1990).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 286
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Citations
[1] Egan, S. and Schleif, R., 1993. A Regulatory Cascade in the Induction of rhaBAD. Journal of Molecular Biology, 234(1), pp.87-98.
[2] Tobin, J. and Schleif, R., 1990. Purification and properties of RhaR, the positive regulator of the l-rhamnose operons of Escherichia coli. Journal of Molecular Biology, 211(1), pp.75-89.
| None |