Part:BBa_K4170030

T7 promoter

Promoter for bacteriophage T7 RNA polymerase

Usage and Biology

This promoter is recognised by T7 RNA polymerase and display constitutive activity which is dependent on the availability of RNA polymerase (Kesik-Brodacka et al., 2012). According to literature, the promoters of the T7 group display high activity and orthogonality to other bacterial promoters which are regulated by bacterial RNA polymerases. This promoter can be used for the in vitro transcription with T7 RNA Polymerase of sequences located downstream of the promoter,ensuring strong transcription and high RNA yields (Conrad et al., 2020).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Citations

[1] Kesik-Brodacka, M., Romanik, A., Mikiewicz-Sygula, D., Plucienniczak, G. and Plucienniczak, A., 2012. A novel system for stable, high-level expression from the T7 promoter. Microbial Cell Factories, 11(1), p.109.

[2] Conrad, T., Plumbom, I., Alcobendas, M., Vidal, R. and Sauer, S., 2020. Maximizing transcription of nucleic acids with efficient T7 promoters. Communications Biology, 3(1).