Part:BBa_K4170001

bdSUMO solubility tag

SUMO tag derived from Brachypodium distachyon

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 289
  
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and biology

SUMO (small ubiquitin modifying protein) protein derived from Brachypodium distachyon which is frequently employed to enhance recombinant protein expression and purification efficiency (Butt, Edavettal, Hall and Mattern, 2005). The tagging system with bdSUMO can be efficiently used for protein production in prokaryotic hosts. On the C-terminus of the bdSUMO coding sequence there is a Gly-Gly motif that is required for SUMO- conjugation to the desired protein. After successful protein expression and purification, a protease (e.g. bdSENP1) which cleaves isopeptide bonds can be used to remove the SUMO tag from the acceptor protein (Frey and Görlich, 2014).

Citations

Butt, T., Edavettal, S., Hall, J. and Mattern, M., 2005. SUMO fusion technology for difficult-to-express proteins. Protein Expression and Purification, 43(1), pp.1-9.

Frey, S. and Görlich, D., 2014. A new set of highly efficient, tag-cleaving proteases for purifying recombinant proteins. Journal of Chromatography A, 1337, pp.95-105.