Composite

Part:BBa_K4169004

Designed by: Jie Min   Group: iGEM22_HZAU-China   (2022-09-30)


J23109+B0033+signal peptide PelB with propeptide and mature nattokinase gene

The expression of Nattokinase in Escherichia coli is prone to the formation of inclusion bodies, so we use weak promoter and RBS combination to express Nattokinase. In order to have enough time and space to fold and secrete nattokinase into the intestine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 262
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 114
    Illegal AgeI site found at 745
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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