Coding

Part:BBa_K4164026

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-30)


CadC-3*GS Linker-FXR.

This part includes the CadC domain fused by a 3*GS Linker to the FXR. It can form a heterodimer with RXRα-CadC, which allows for the binding of the pCadBA operon region and promotes the transcription of GFP.

The CadC domain was from BBa_K3425100 and a flexible linker (3*GGGGS) was added between CadC and FXR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1639


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Categories
Parameters
None