Composite

Part:BBa_K4164016

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-30)


Inductive expression of ddRFPA1-ddRFPB1

This composite part was constructed to analyze the function of ddRFP and the intensity of the T7 lac promoter.

The composite part can be directly imported into plasmid and express ddRFPA1-ddRFPB1 induced with IPTG.

We connected ddRFPA1 and ddRFPB1 by a flexible linker(3*GGGGS) to verify the feasicility of ddRFPA1 and ddRFPB1. Following overnight incubation at 37℃, we can see the bright red fluorescence (Fig.1). In this case, we chose ddRFPA1 and ddRFPB1 as our report device.


Fig.1 Fluorescence image of E. coli expressing ddRFPA1-ddRFPB1 and control.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1271
    Illegal NheI site found at 1539
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 830
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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