Part:BBa_K4164011

constitutive expression of FXR-ddRFPA1 and RXR-ddRFPB1

In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried low intensity promoter at first. This composite part contains the low intensity promoter (BBa_J23105) and RBS. The composite part can be directly imported into plasmid and express FXR-ddRFPA1 and RXR-ddRFPB1 at the same time.

We conducted a set of CDCA concentration gradient experiments to measure its fluorescence intensity. The data showed that the fluorescence intensity changed very little under the activation of different concentrations of CDCA. Consistently, no significant differences were observed.

Fig.1 Fluorescence intensity/OD using a gradient of CDCA concentrations based on ddRFP..

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 2284
  Illegal NheI site found at 2307
  Illegal NheI site found at 4199
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1533
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2689
  Illegal AgeI site found at 3716
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3071
  Illegal SapI.rc site found at 1122