Composite

Part:BBa_K4164008

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-30)


constitutive expression of BSS

In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried low intensity promoter at first.This composite part contains the low intensity promoter (BBa_J23105) and RBS. The composite part can be directly imported into plasmid and express BSS. If you want to learn about the detailed introduction of BSS, you can click the link below. https://parts.igem.org/Part:BBa_K4164001

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 965
    Illegal NgoMIV site found at 1016
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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