DNA

Part:BBa_K4162024

Designed by: Weiwen Chen   Group: iGEM22_Fudan   (2022-10-02)


ribozyme + T7_RBS + BCMO + ribozyme + T7_RBS + ybbO

Introduction

2022 Fudan

BCMO and gene ybbO are assembled together and separated by DNA sequence of hammerhead ribozyme Part:BBa_K4162005. Due to the self-cleaving of hammerhead ribozyme, mRNA transcribed from the two coding sequences is divided into two segments, corresponding to BCMO and ybbO respectively, so as to prevent the translation process of the two enzyme sequences from interfering with each other.

This biobrick was later enhanced to support TEARS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1018
    Illegal AgeI site found at 1611
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None