Part:BBa_K4162004

Gene coding for BCMO from uncultured marine bacteria

Introduction

Retinal can be produced by introducing the β-carotene mono(di)oxygenase (BCMO) gene into recombinant E. coli that produces β-carotene. This codon-optimized BCMO gene is based on the amino acid sequence of the uncultured marine bacterium 66A03 blh gene.^[1]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

↑ Jang, Hui-Jeong et al. “Retinoid production using metabolically engineered E. coli with a two-phase culture system.” Microbial cell factories vol. 10 59. 29 Jul. 2011, doi:10.1186/1475-2859-10-59

[1] Jang, Hui-Jeong et al. “Retinoid production using metabolically engineered E. coli with a two-phase culture system.” Microbial cell factories vol. 10 59. 29 Jul. 2011, doi:10.1186/1475-2859-10-59

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