Part:BBa_K4162001

StayGold = (n2)oxStayGold(c4)v2.0

Introduction

A new version of StayGold^[1], which gives a higher expression level than the original version, and is over one order of magnitude more photostable than EGFP. The StayGold gene has been codon optimization for exogenous expression.

Worth to Notice

This biobrick is the version of StayGold available on Addgene.

Characterization

Figure 1. Fluorescent imaging confirm the expression of both BCMO and ybbO. We observed fluorescence images of E. coli (1. Bacteria expressing BCMO-linker-StayGold ; 2. Bacteria expressing BCMO and ybbO-tdMCP-GFP ) through an Olympus fluorescence microscope with a 1.45 NA 150× oil objective. GFP and StayGold were excited by a 488 nm laser. Images were taken by the same settings for comparison.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 638
Illegal BsaI.rc site found at 658

↑ A highly photostable and bright green fluorescent protein. Hirano M, Ando R, Shimozono S, Sugiyama M, Takeda N, Kurokawa H, Deguchi R, Endo K, Haga K, Takai-Todaka R, Inaura S, Matsumura Y, Hama H, Okada Y, Fujiwara T, Morimoto T, Katayama K, Miyawaki A. Nat Biotechnol, 2022 Jul;40(7):1132-1142. PMID:35468954

[1] A highly photostable and bright green fluorescent protein. Hirano M, Ando R, Shimozono S, Sugiyama M, Takeda N, Kurokawa H, Deguchi R, Endo K, Haga K, Takai-Todaka R, Inaura S, Matsumura Y, Hama H, Okada Y, Fujiwara T, Morimoto T, Katayama K, Miyawaki A. Nat Biotechnol, 2022 Jul;40(7):1132-1142. PMID:35468954

[1]