Composite

Part:BBa_K4161997

Designed by: Zhujun Yao 姚竹君   Group: iGEM22_DKU   (2022-10-10)


Usp45-signalling peptide in L.lactis-20ipaD-AcmA3b

The composite part can be added within a vector and expressed in L.lactis using the NICE system. The function of the composite part is to let L.lactis display 20ipaD on the cell wall. Usp45 is the nisin-inducible promoter, the signaling peptide helps the protein to anchor on the cell wall, and the AcmA3b is the protein that interacts with the cell wall by non-covalent bonds. With 20ipaD fused at the N-terminal of AcmA3b, the nanobody can be displayed at L.lactis surface.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 591
    Illegal SpeI site found at 1137
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 591
    Illegal SpeI site found at 1137
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 591
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 591
    Illegal SpeI site found at 1137
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 591
    Illegal SpeI site found at 1137
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None