Protein_Domain

Part:BBa_K4161504

Designed by: Lingrui Lai   Group: iGEM22_DKU   (2022-07-26)


15aa, (G4S)3 linker

A flexible and protease-resistant linker sequence comprises a triplicate of the "Gly-Gly-Gly-Gly-Ser" repeat.

Usage and Biology

Biology

Glycine provides the whole linker sequence enough flexibility while serine provide some rigidity.

Usage

Linker sequences are used to connect proteins for producing recombinant proteins or to reduce steric hindrance between various protein domains.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None