Coding

Part:BBa_K4161503

Designed by: Lingrui Lai   Group: iGEM22_DKU   (2022-07-26)


68aa, Aga2 subunit

This DNA sequence expresses the Aga2 subunit that resides on the extracellular side of the S. cerevisiae's plasmic membrane.

Usage and Biology

Biology

Aga2 protein subunit connects to another subunit of a-agglutinin named Aga1 that anchors on the membrane of S. cerevisiae through a disulfide bond. It faces the extracellular side of the membrane.

Usage

When connected to your interest gene this sequence can be used to express a fused protein of your interest protein and the Aga2 protein that enables you to display your target protein on the surface of S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 268
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 268
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 268
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
Parameters
None