DNA

Part:BBa_K4161502

Designed by: Lingrui Lai   Group: iGEM22_DKU   (2022-07-25)


A promoter that allow the plasmid to be expressed in S. cerevisiae

This promoter enables the plasmid to inducibly express proteins in S. cerevisiae by adding galactose.

Usage and Biology

Biology

GAL1 encodes for an enzyme specific to galactose metabolism--galactokinase. GAL1 promoter can be repressed by glucose or induced by galactose.

Usage

GAL1 promoter is one of the strongest promoters used in S. cerevisiae for recombinant protein production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
None