Protein_Domain

Part:BBa_K4161500

Designed by: Yuxin Wang   Group: iGEM22_DKU   (2022-10-13)


20ipaD for S. cereviae Expression

This part encodes for a single-domain antibody, or nanobody, called 20ipaD. The nanobody showed high binding affinity against an important epitope of pathogenic Shigella. The amino acid sequence of this nanobody was retrieved from the 2017 paper of Barta et al, and DNA sequence was optimized for Saccharomyces cerevisiae expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None