Plasmid_Backbone

Part:BBa_K4161302

Designed by: Qizhou Jiang   Group: iGEM22_DKU   (2022-07-25)


pyD1 plasmid

This empty backbone expression vector is designed for Saccharomyces cerevisiae cells, which can be used for yeast surface display. The AmpR gene can be used to detect whether your yeasts are successfully transformed. You can insert genes into the sequence between T7 promoter and 6xHis since the terminator is behind 6xHis. In order to propagate and maintain pyD1, you can transform the plasmids into E.coli for further storage.

Detailed information: File:Pyd1 man.pdf

PYD1.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 936
    Illegal XbaI site found at 975
    Illegal XbaI site found at 2019
    Illegal PstI site found at 801
    Illegal PstI site found at 941
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 936
    Illegal NheI site found at 858
    Illegal PstI site found at 801
    Illegal PstI site found at 941
    Illegal NotI site found at 962
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 936
    Illegal BamHI site found at 921
    Illegal XhoI site found at 969
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 936
    Illegal XbaI site found at 975
    Illegal XbaI site found at 2019
    Illegal PstI site found at 801
    Illegal PstI site found at 941
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 936
    Illegal XbaI site found at 975
    Illegal XbaI site found at 2019
    Illegal PstI site found at 801
    Illegal PstI site found at 941
    Illegal AgeI site found at 71
    Illegal AgeI site found at 1038
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1020
    Illegal BsaI site found at 3646
    Illegal SapI.rc site found at 2624
    Illegal SapI.rc site found at 4728


Source

The plasmid backbone of pyD1 plasmid is the product of Invitrogen Company.

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