Plasmid_Backbone
Part:BBa_K4161302
Designed by: Qizhou Jiang Group: iGEM22_DKU (2022-07-25)
pyD1 plasmid
This empty backbone expression vector is designed for Saccharomyces cerevisiae cells, which can be used for yeast surface display. The AmpR gene can be used to detect whether your yeasts are successfully transformed. You can insert genes into the sequence between T7 promoter and 6xHis since the terminator is behind 6xHis. In order to propagate and maintain pyD1, you can transform the plasmids into E.coli for further storage.
Detailed information: File:Pyd1 man.pdf
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 936
Illegal XbaI site found at 975
Illegal XbaI site found at 2019
Illegal PstI site found at 801
Illegal PstI site found at 941 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 936
Illegal NheI site found at 858
Illegal PstI site found at 801
Illegal PstI site found at 941
Illegal NotI site found at 962 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 936
Illegal BamHI site found at 921
Illegal XhoI site found at 969 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 936
Illegal XbaI site found at 975
Illegal XbaI site found at 2019
Illegal PstI site found at 801
Illegal PstI site found at 941 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 936
Illegal XbaI site found at 975
Illegal XbaI site found at 2019
Illegal PstI site found at 801
Illegal PstI site found at 941
Illegal AgeI site found at 71
Illegal AgeI site found at 1038 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1020
Illegal BsaI site found at 3646
Illegal SapI.rc site found at 2624
Illegal SapI.rc site found at 4728
Source
The plasmid backbone of pyD1 plasmid is the product of Invitrogen Company.
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Categories
Parameters
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