Plasmid_Backbone

Part:BBa_K4161025

Designed by: Yunyi Ru   Group: iGEM22_DKU   (2022-10-10)


pUC19

This vector is used for recombinant plasmid construction in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 683
    Illegal XbaI site found at 656
    Illegal PstI site found at 644
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 683
    Illegal PstI site found at 644
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 683
    Illegal BamHI site found at 662
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 683
    Illegal XbaI site found at 656
    Illegal PstI site found at 644
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 683
    Illegal XbaI site found at 656
    Illegal PstI site found at 644
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1999
    Illegal SapI.rc site found at 395


[edit]
Categories
Parameters
None