Other

Part:BBa_K4161023

Designed by: Yunyi Ru   Group: iGEM22_DKU   (2022-10-10)


csgA-JPS-G3-3xFLAG

This part aims to display the recombinant protein JPS-G3-3xFLAG on the surface of E. coli, and the curli fiber protein csgA is used as the surface display system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 818
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 18
    Illegal PstI site found at 818
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 818
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 818
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None