Coding

Part:BBa_K4161002

Designed by: Chang Shen   Group: iGEM22_DKU   (2022-07-20)


JMK-H2

JMK-H2 is a VHH antibody wich targets the IpaD antigen that makes up the needle tip structure of Type III secretion system of Shigella. The DNA sequence is reversed from the JMK-H2 protein sequence and codon optimized for expression in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The sequence can be accessed through RCSB PDB with PDB ID 5VXK. (https://www.rcsb.org/structure/5VXK)

Reference

Barta, Michael L., Jonathan P. Shearer, Olivia Arizmendi, Jacqueline M. Tremblay, Nurjahan Mehzabeen, Qi Zheng, Kevin P. Battaile, et al. “Single-Domain Antibodies Pinpoint Potential Targets within Shigella Invasion Plasmid Antigen D of the Needle Tip Complex for Inhibition of Type III Secretion.” Journal of Biological Chemistry 292, no. 40 (October 2017): 16677–87. https://doi.org/10.1074/jbc.M117.802231.

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Parameters
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