Composite

Part:BBa_K416007

Designed by: Russell Durrett   Group: iGEM10_NYU   (2010-10-15)


LexO:pCYC:RBS

This composite part consists of the Zif268 operator : the Lex operator : the pCYC minimal promoter and a Ribosome Binding Sequence (Kozak sequence).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 453
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 453
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
    Illegal XhoI site found at 41
    Illegal XhoI site found at 203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 453
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 453
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None