Part:BBa_K4160016

STAT-induced SEAP

This composite part encodes for STAT-induced SEAP and is composed of the basic parts STAT promotor (BBa_K4160006) and SEAP (BBa_K1470004).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 209
  Illegal XbaI site found at 233
  Illegal PstI site found at 53
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 209
  Illegal PstI site found at 53
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 209
  Illegal BamHI site found at 227
  Illegal BamHI site found at 459
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 209
  Illegal XbaI site found at 233
  Illegal PstI site found at 53
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 209
  Illegal XbaI site found at 233
  Illegal PstI site found at 53
  Illegal NgoMIV site found at 770
  Illegal NgoMIV site found at 1739
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1100
  Illegal BsaI.rc site found at 1589
  

Usage & biology

TU-Eindhoven 2022 used this composite part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system that is developed by Scheller et al., 2018.¹ Upon activation of the GEMS receptor, STAT-induced SEAP will be expressed and secreted via the JAK/STAT signaling pathway. In the project of TU-Eindhoven 2022, SEAP was used as a genetic reporter protein to investigate the activation of the GEMS receptor (BBa_K4160008, BBa_K4160009, BBa_K4160010, BBa_K4160012, and BBa_K4160013). STAT-induced SEAP was expressed in a pLS13 plasmid.

Characterization

Characterization of STAT-induced SEAP expression by the GEMS system can be found on the BBa_K4160008 page.

References

1. Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M. Generalized extracellular molecule sensor platform for programming cellular behavior. Nat Chem Biol. Published online 2018. doi:10.1038/s41589-018-0046-z